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Plant Physiology文章:ARF3参与芽分生组织诱导过程中生长素与细胞分裂素之间的调控反应

日期:2019/03/18  来源:新葡萄京娱乐场官方网站

  

        植物的器官再生系统广泛地应用于干细胞诱导、细胞命运决定、激素信号等方面的基础研究中。尽管人们普遍认同植物器官的再生过程受到生长素与细胞分裂素的共同调控,但是生长素与细胞分裂素之间潜在的分子机制仍未被阐明。编辑研究了拟南芥芽部分生组织诱导的过程,发现局部生物合成与极性运输使得生长素的梯度分布具有时空特异性,从而决定了细胞分裂素的反应。生长素和细胞分裂素之间的这种关联在分生组织形成,芽部再生的过程中十分重要。运用遗传学或者药理学手段对生长素分布进行干预后,细胞分裂素的反应以及AtIPT5的表达被阻断,从而影响了干细胞的诱导与分生组织的形成。编辑采用Affymetrix拟南芥表达谱芯片进行转录本分析,Arabidopsis ATH1 Genome Array检测服务北京新葡萄京娱乐场官方网站生物技术有限企业完成。研究发现转录因子ARF3参与介导器官再生过程中的细胞分裂素反应。同时,研究发现ARF3的突变会导致AtIPT5的异常表达。此外,ARF3可以直接与AtIPT5的启动子结合,从而负调控AtIPT5的表达。因此,ARF3通过调控AtIPT5的表达可以造成细胞分裂素生物合成异常,最终导致器官再生被阻断。这篇研究阐述了生长素和细胞分裂素反应之间的分子机制并揭示了此过程中参与介导调控的信号因子。


原文摘要:

     Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from the Regulation of Cytokinin Biosynthesis by AUXIN RESPONSE FACTOR3

     De novo organ regeneration is an excellent biological system for the study of fundamental questions regarding stem cell initiation, cell fate determination, and hormone signaling. Despite the general belief that auxin and cytokinin responses interact to regulate de novo organ regeneration, the molecular mechanisms underlying such a cross talk are little understood. Here, we show that spatiotemporal biosynthesis and polar transport resulted in local auxin distribution in Arabidopsis (Arabidopsis thaliana), which in turn determined the cytokinin response during de novo shoot regeneration. Genetic and pharmacological interference of auxin distribution disrupted the cytokinin response and ATP/ADP ISOPENTENYLTRANSFERASE5 (AtIPT5) expression, affecting stem cell initiation and meristem formation. Transcriptomic data suggested that AUXIN RESPONSE FACTOR3 (ARF3) mediated the auxin response during de novo organ regeneration. Indeed, mutations in ARF3 caused ectopic cytokinin biosynthesis via the misexpression of AtIPT5, and this disrupted organ regeneration. We further showed that ARF3 directly bound to the promoter of AtIPT5 and negatively regulated AtIPT5 expression. The results from this study thus revealed an auxin-cytokinin cross talk mechanism involving distinct intermediate signaling components required for de novo stem cell initiation and shed new light on the mechanisms of organogenesis in planta.

原文出处:http://www.plantphysiol.org/content/161/1/240

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